Journal: Stem Cell Research & Therapy

Article Title: Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro

doi: 10.1186/s13287-018-0876-3

Figure Lengend Snippet: Surface marker expression of in-vitro aging MSCs. MSCs were cultivated with either DMEM-based ( a ) or αMEM-based ( b ) expansion media and were analyzed by flow cytometry for the expression of specific MSC positive (Stro-1, CD29, CD44, CD73, CD90, CD105, CD106, CD 146) and negative (CD34, CD45) surface antigens. n = 4–6; * p < 0.05, versus the corresponding P8-MSCs cultured under the same expansion condition within the same surface marker analysis. αMEM, minimum essential medium alpha; DMEM, Dulbecco’s modified Eagle’s medium

Article Snippet: Specifically, antibodies against Stro-1 (ab190282) and CD73 (ab106677) were purchased from Abcam (Cambridge, MA, USA); CD29 (MCA1949A647), CD34 (MCA547PE), CD44 (MCA89PE), and CD106 (MCA907F) were from Bio-Rad (Kidlington, Oxford, UK); and CD45, CD90, CD105, and CD146 (FM002) were from R&D Systems (Minneapolis, MN, USA).

Techniques: Marker, Expressing, In Vitro, Flow Cytometry, Cell Culture, Modification

Journal: Cells

Article Title: Methylglyoxal Impairs the Pro-Angiogenic Ability of Mouse Adipose-Derived Stem Cells (mADSCs) via a Senescence-Associated Mechanism

doi: 10.3390/cells12131741

Figure Lengend Snippet: mADSCs characterization. ( a ) Representative photographs (5× magnification of an optical microscope, scale bar 500 µm) of mADSCs isolated from the subcutaneous adipose tissue of C57bl6 mice cultured in selection medium for 4 passages. ( b ) Accumulated cell number and ( c ) population doubling time (PTD) per hours of mADSCs from p3 to p11. Once 90% confluence was reached, cells were counted by an automated cell counter. Dots in line graphs represent the mean ± SEM values. ( d – i ) Representative histograms of the expression of CD44, CD29, CD90.2, Sca-1, CD45 and CD31 surface markers analyzed by FACS.

Article Snippet: Then, the cells were divided into 4 aliquots: (i) stained with APC-labeled anti-CD29 (Miltenyi Biotec 130-119-166), FITC-labeled anti-CD45 (Miltenyi Biotec 130-110-796) and PE-labeled anti-CD90.2 (Miltenyi Biotec 130-120-897) in 100 μL of PBS; (ii) PE-labeled anti-CD44 and APC-labeled anti-CD31 antibodies in 100 μL of PBS; (iii) APC-labeled anti-Sca-1 (Miltenyi Biotec 130-123-848) in 100 μL of PBS; or (iv) used as negative control.

Techniques: Microscopy, Isolation, Cell Culture, Selection, Expressing