Journal: Cells

Article Title: Methylglyoxal Impairs the Pro-Angiogenic Ability of Mouse Adipose-Derived Stem Cells (mADSCs) via a Senescence-Associated Mechanism

doi: 10.3390/cells12131741

Figure Lengend Snippet: mADSCs characterization. ( a ) Representative photographs (5× magnification of an optical microscope, scale bar 500 µm) of mADSCs isolated from the subcutaneous adipose tissue of C57bl6 mice cultured in selection medium for 4 passages. ( b ) Accumulated cell number and ( c ) population doubling time (PTD) per hours of mADSCs from p3 to p11. Once 90% confluence was reached, cells were counted by an automated cell counter. Dots in line graphs represent the mean ± SEM values. ( d – i ) Representative histograms of the expression of CD44, CD29, CD90.2, Sca-1, CD45 and CD31 surface markers analyzed by FACS.

Article Snippet: CD29-APC, CD44-PE, CD45-FITC, CD31-APC, CD90.2-PE and Sca-1-APC antibodies were provided by Miltenyi Biotec (Auburn, CA, USA).

Techniques: Microscopy, Isolation, Cell Culture, Selection, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro

doi: 10.1186/s13287-018-0876-3

Figure Lengend Snippet: Surface marker expression of in-vitro aging MSCs. MSCs were cultivated with either DMEM-based ( a ) or αMEM-based ( b ) expansion media and were analyzed by flow cytometry for the expression of specific MSC positive (Stro-1, CD29, CD44, CD73, CD90, CD105, CD106, CD 146) and negative (CD34, CD45) surface antigens. n = 4–6; * p < 0.05, versus the corresponding P8-MSCs cultured under the same expansion condition within the same surface marker analysis. αMEM, minimum essential medium alpha; DMEM, Dulbecco’s modified Eagle’s medium

Article Snippet: Specifically, antibodies against Stro-1 (ab190282) and CD73 (ab106677) were purchased from Abcam (Cambridge, MA, USA); CD29 (MCA1949A647), CD34 (MCA547PE), CD44 (MCA89PE), and CD106 (MCA907F) were from Bio-Rad (Kidlington, Oxford, UK); and CD45, CD90, CD105, and CD146 (FM002) were from R&D Systems (Minneapolis, MN, USA).

Techniques: Marker, Expressing, In Vitro, Flow Cytometry, Cell Culture, Modification

Journal: PLoS ONE

Article Title: Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation

doi: 10.1371/journal.pone.0038917

Figure Lengend Snippet: Mice were challenged with LPS via inhalation and sacrificed 4 hours later. In addition, neutrophils were depleted by antibody injection or mice were treated with simvastatin (2 µg/g bodyweight) 12 hours and one hour before or one hour after LPS exposure as indicated. A: Quantification of alveolar (top), interstitial (middle), and intravascular neutrophils (bottom) in mice treated as indicated. B: FITC-dextran clearance (top), albumin concentration (middle), and elastase activity (bottom) in BAL fluids in mice treated as indicated (n = 8–10 for each bar). Statistical significance was tested using one way ANOVA with Newman-Keuls Multiple Comparison test. * indicates significant difference compared to LPS-treated animals.

Article Snippet: Cell pellets were labeled with PerCP-Cy5.5 anti-mouse Ly-6G, PE anti-mouse CD115, APC-Cy7 anti-mouse CD45 und APC anti-Mouse Ly- 6G (all eBioscience) and FITC anti-mouse CD29 (Miltenyi Biotec).

Techniques: Injection, Concentration Assay, Activity Assay, Comparison

Journal: PLoS ONE

Article Title: Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation

doi: 10.1371/journal.pone.0038917

Figure Lengend Snippet: Isolated human neutrophils were pre-treated with Simvastatin (3 hours, 1 or 10 µM) and then activated with fMLP. MFI of surface expression of CD11b (A), CD29 (B) and FPRL1 (C) as measured by flow cytometry after staining with directly conjugated antibodies (n = 3–6; repetition = 4). D+E In vivo degranulation after LPS inhalation. Mice were challenged with LPS via inhalation and sacrificed 4 hours later. After isolation of neutrophils from the blood and the lung, MFI of surface expression of CD11b and CD29 as measured by flow cytometry after staining with directly conjugated antibodies (n = 4–6). Statistical significance was tested using one way ANOVA with Newman-Keuls Multiple Comparison test. * indicates significant difference in comparison to the fMLP or the LPS group.

Article Snippet: Cell pellets were labeled with PerCP-Cy5.5 anti-mouse Ly-6G, PE anti-mouse CD115, APC-Cy7 anti-mouse CD45 und APC anti-Mouse Ly- 6G (all eBioscience) and FITC anti-mouse CD29 (Miltenyi Biotec).

Techniques: Isolation, Expressing, Flow Cytometry, Staining, In Vivo, Comparison

Journal: Biomedical Reports

Article Title: Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood

doi: 10.3892/br.2019.1236

Figure Lengend Snippet: Flow cytometry antibody panel used for characterization.

Article Snippet: Supplier CD29 PE HMs1-1 130-102-994 Miltenyi Biotec, Inc. CD44 FITC IM7.8.1 130-102-511 Miltenyi Biotec, Inc. CD105 PE MJ7/18 130-102-548 Miltenyi Biotec, Inc. CD90.2 FITC 30-H12 130-120-091 Miltenyi Biotec, Inc. CD146 FITC ME-9F1 130-102-230 Miltenyi Biotec, Inc. SCa-1 PE D7 130-102-832 Miltenyi Biotec, Inc. CD45 FITC 30F11.1 130-110-658 Miltenyi Biotec, Inc. CD140b PE APB5 130-118-457 Miltenyi Biotec, Inc. Open in a separate window CD, cluster of differentiation; PE, phycoethrin; FITC, fluorescein isothiocyanate.

Techniques: Flow Cytometry

Journal: Biomedical Reports

Article Title: Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood

doi: 10.3892/br.2019.1236

Figure Lengend Snippet: Flow cytometric analysis of BM-MSCs and PB-MSCs. BM-MSCs were positive CD29 and negative for other markers, while PB-MSCs were positive for CD146, CD29, and CD140b and negative for Sca-1, CD44, CD45, CD90 and CD105. CD, cluster of differentiation; BM-MSCs, bone marrow-mesenchymal stromal cells; PB, peripheral blood; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Article Snippet: Supplier CD29 PE HMs1-1 130-102-994 Miltenyi Biotec, Inc. CD44 FITC IM7.8.1 130-102-511 Miltenyi Biotec, Inc. CD105 PE MJ7/18 130-102-548 Miltenyi Biotec, Inc. CD90.2 FITC 30-H12 130-120-091 Miltenyi Biotec, Inc. CD146 FITC ME-9F1 130-102-230 Miltenyi Biotec, Inc. SCa-1 PE D7 130-102-832 Miltenyi Biotec, Inc. CD45 FITC 30F11.1 130-110-658 Miltenyi Biotec, Inc. CD140b PE APB5 130-118-457 Miltenyi Biotec, Inc. Open in a separate window CD, cluster of differentiation; PE, phycoethrin; FITC, fluorescein isothiocyanate.

Techniques:

Journal: bioRxiv

Article Title: Evaluation of M2-like macrophage enrichment after diffuse traumatic brain injury through transient interleukin-4 expression from engineered mesenchymal stromal cells

doi: 10.1101/2020.01.28.918441

Figure Lengend Snippet: (A) MSCs (Cyagen) used in this study expressed CD29, CD44, and Sca-1 while remaining negative for CD31 and CD45 in at least two late passage numbers.

Article Snippet: For MSC cell-surface characterization, the following antibodies were used: APC-Vio770 CD29 (Miltenyi Biotec; clone: HMβ1-1), VioBright FITC CD44 (Miltenyi Biotec, US; clone: REA665), PerCP Sca-1 (Biolegend, US; clone: D7), PE CD31 (Miltenyi Biotec, US; clone: 390), PE CD45 (Biolegend, US; clone: 30-F11), and PE-Vio770 CD105 (Miltenyi Biotec, US; clone: MJ7/19).

Techniques: